The second cell line (IM-1) was from a biopsy of lymphoid tissue (lingual tonsil) from a 54-year-old white female with diagnosis of lymphoblastic lymphoma and cutaneous lymphoma

remains unknown, however, whether the original cells in cul ture synthesized immunoglobulins or whether this capacity de veloped in the culture environment. The successful establish rnent of continuous white cell cultures from two patients who are still alive has given us an opportunity to investigate this question. Morphologic, immunochemical, ixnmunofluorescent, and bio synthetic methods were used to compare freshly obtained tis sue with the cell lines established and growing in tissue cul.. ture.